Mojgan Daneshkhah; Mahbubeh Setorki
Volume 21, Issue 2 , 2019, Pages 1-8
Abstract
Background: A new approach in the treatment of epilepsy is to use new drugs with neuroprotective, antioxidant, and anti-inflammatory effects.Objectives: The study aimed to investigate the protective effects of Artemisia persica essential oil (EO) against pentylenetetrazol(PTZ)-induced seizure in mice.Methods: ...
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Background: A new approach in the treatment of epilepsy is to use new drugs with neuroprotective, antioxidant, and anti-inflammatory effects.Objectives: The study aimed to investigate the protective effects of Artemisia persica essential oil (EO) against pentylenetetrazol(PTZ)-induced seizure in mice.Methods: This experimental study was conducted at the Izeh Islamic Azad University, Iran. 70 male BALB/c mice were divided intoseven groups of 10 using simple random allocation, including control (normal saline), PTZ (35 mg/kg i.p. with 48 hours intervalsand then 60 mg/kg on the 10th day), interventions (PTZ plus daily i.p. injection of EO at doses of 50, 75, and 100 mg/kg), diazepam(PTZ plus EO at a dose of 100 mg/kg + diazepam), and flumazenil (PTZ plus EO at a dose of 100 mg/kg + flumazenil) groups.Results: The treatment of PTZ-kindled mice with 50 mg/kg of EO significantly reduced the seizure onset latency (P < 0.05). EO ata dose of 100 mg/kg significantly decreased tonic seizures, head tics, and repeated spinning and jumping (P < 0.05). Diazepamimproved, and flumazenil weakened the anticonvulsant effects of the EO. The treatment of PTZ-kindled mice with EO (100 mg/kg)significantly decreased nitric oxide and malondialdehyde, and increased the total antioxidant capacity in both serum and brain (P< 0.05). EO at a dose of 100 mg/kg could significantly decrease IL-1β and TNF-α expression in the brain of epileptic mice (P < 0.05).Conclusions: A. persica EO shows anticonvulsant effects through benzodiazepine receptor binding activity and modulation of ox-idative stress and the inflammatory process.
